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cleaved caspase 9 asp315 rabbit pab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 9 asp315 rabbit pab
    Effects of SAR405 on apoptosis of H2452 pleural mesothelioma cells. (A) Mesothelioma H2452 cells were treated with 10.0 μM SAR405 for 48 h, stained with Hoechst 33342 to assess nuclear morphology, and visualized under a fluorescence microscope. Arrows indicate apoptotic cells. Scale bars = 200 μm. (B) Mesothelioma cells were treated with 2.5–10.0 μM SAR405 or dimethyl sulfoxide (DMSO) for 24 h and subjected to western blotting analysis to determine the expression of poly ADP‐ribose polymerase (PARP), cleaved (c‐) PARP, caspase‐3, c‐caspase‐3, and <t>c‐caspase‐9.</t> GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase. (C) The induction of apoptosis by SAR405 was evaluated using flow cytometry. Apoptotic cells were stained with annexin‐V/fluorescein isothiocyanate (FITC) and propidium iodide (PI). Data are presented as the mean ± SD of three independent experiments. * p < 0.05 compared with DMSO.
    Cleaved Caspase 9 Asp315 Rabbit Pab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 469 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+cleaved+caspase+9+antibody/Cleaved+Caspase-9+(Asp315)+Antibody/pmc12952911-47-16-44
    Average 96 stars, based on 469 article reviews
    cleaved caspase 9 asp315 rabbit pab - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Effects of Autophagy Inhibition by SAR405 , a Selective VPS34 Inhibitor, on Pleural Mesothelioma Cells"

    Article Title: Effects of Autophagy Inhibition by SAR405 , a Selective VPS34 Inhibitor, on Pleural Mesothelioma Cells

    Journal: Thoracic Cancer

    doi: 10.1111/1759-7714.70255

    Effects of SAR405 on apoptosis of H2452 pleural mesothelioma cells. (A) Mesothelioma H2452 cells were treated with 10.0 μM SAR405 for 48 h, stained with Hoechst 33342 to assess nuclear morphology, and visualized under a fluorescence microscope. Arrows indicate apoptotic cells. Scale bars = 200 μm. (B) Mesothelioma cells were treated with 2.5–10.0 μM SAR405 or dimethyl sulfoxide (DMSO) for 24 h and subjected to western blotting analysis to determine the expression of poly ADP‐ribose polymerase (PARP), cleaved (c‐) PARP, caspase‐3, c‐caspase‐3, and c‐caspase‐9. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase. (C) The induction of apoptosis by SAR405 was evaluated using flow cytometry. Apoptotic cells were stained with annexin‐V/fluorescein isothiocyanate (FITC) and propidium iodide (PI). Data are presented as the mean ± SD of three independent experiments. * p < 0.05 compared with DMSO.
    Figure Legend Snippet: Effects of SAR405 on apoptosis of H2452 pleural mesothelioma cells. (A) Mesothelioma H2452 cells were treated with 10.0 μM SAR405 for 48 h, stained with Hoechst 33342 to assess nuclear morphology, and visualized under a fluorescence microscope. Arrows indicate apoptotic cells. Scale bars = 200 μm. (B) Mesothelioma cells were treated with 2.5–10.0 μM SAR405 or dimethyl sulfoxide (DMSO) for 24 h and subjected to western blotting analysis to determine the expression of poly ADP‐ribose polymerase (PARP), cleaved (c‐) PARP, caspase‐3, c‐caspase‐3, and c‐caspase‐9. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase. (C) The induction of apoptosis by SAR405 was evaluated using flow cytometry. Apoptotic cells were stained with annexin‐V/fluorescein isothiocyanate (FITC) and propidium iodide (PI). Data are presented as the mean ± SD of three independent experiments. * p < 0.05 compared with DMSO.

    Techniques Used: Staining, Fluorescence, Microscopy, Western Blot, Expressing, Flow Cytometry

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    Effects of SAR405 on apoptosis of H2452 pleural mesothelioma cells. (A) Mesothelioma H2452 cells were treated with 10.0 μM SAR405 for 48 h, stained with Hoechst 33342 to assess nuclear morphology, and visualized under a fluorescence microscope. Arrows indicate apoptotic cells. Scale bars = 200 μm. (B) Mesothelioma cells were treated with 2.5–10.0 μM SAR405 or dimethyl sulfoxide (DMSO) for 24 h and subjected to western blotting analysis to determine the expression of poly ADP‐ribose polymerase (PARP), cleaved (c‐) PARP, caspase‐3, c‐caspase‐3, and <t>c‐caspase‐9.</t> GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase. (C) The induction of apoptosis by SAR405 was evaluated using flow cytometry. Apoptotic cells were stained with annexin‐V/fluorescein isothiocyanate (FITC) and propidium iodide (PI). Data are presented as the mean ± SD of three independent experiments. * p < 0.05 compared with DMSO.
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    Effects of SAR405 on apoptosis of H2452 pleural mesothelioma cells. (A) Mesothelioma H2452 cells were treated with 10.0 μM SAR405 for 48 h, stained with Hoechst 33342 to assess nuclear morphology, and visualized under a fluorescence microscope. Arrows indicate apoptotic cells. Scale bars = 200 μm. (B) Mesothelioma cells were treated with 2.5–10.0 μM SAR405 or dimethyl sulfoxide (DMSO) for 24 h and subjected to western blotting analysis to determine the expression of poly ADP‐ribose polymerase (PARP), cleaved (c‐) PARP, caspase‐3, c‐caspase‐3, and <t>c‐caspase‐9.</t> GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase. (C) The induction of apoptosis by SAR405 was evaluated using flow cytometry. Apoptotic cells were stained with annexin‐V/fluorescein isothiocyanate (FITC) and propidium iodide (PI). Data are presented as the mean ± SD of three independent experiments. * p < 0.05 compared with DMSO.
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    Effects of SAR405 on apoptosis of H2452 pleural mesothelioma cells. (A) Mesothelioma H2452 cells were treated with 10.0 μM SAR405 for 48 h, stained with Hoechst 33342 to assess nuclear morphology, and visualized under a fluorescence microscope. Arrows indicate apoptotic cells. Scale bars = 200 μm. (B) Mesothelioma cells were treated with 2.5–10.0 μM SAR405 or dimethyl sulfoxide (DMSO) for 24 h and subjected to western blotting analysis to determine the expression of poly ADP‐ribose polymerase (PARP), cleaved (c‐) PARP, caspase‐3, c‐caspase‐3, and <t>c‐caspase‐9.</t> GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase. (C) The induction of apoptosis by SAR405 was evaluated using flow cytometry. Apoptotic cells were stained with annexin‐V/fluorescein isothiocyanate (FITC) and propidium iodide (PI). Data are presented as the mean ± SD of three independent experiments. * p < 0.05 compared with DMSO.
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    Image Search Results


    Effects of SAR405 on apoptosis of H2452 pleural mesothelioma cells. (A) Mesothelioma H2452 cells were treated with 10.0 μM SAR405 for 48 h, stained with Hoechst 33342 to assess nuclear morphology, and visualized under a fluorescence microscope. Arrows indicate apoptotic cells. Scale bars = 200 μm. (B) Mesothelioma cells were treated with 2.5–10.0 μM SAR405 or dimethyl sulfoxide (DMSO) for 24 h and subjected to western blotting analysis to determine the expression of poly ADP‐ribose polymerase (PARP), cleaved (c‐) PARP, caspase‐3, c‐caspase‐3, and c‐caspase‐9. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase. (C) The induction of apoptosis by SAR405 was evaluated using flow cytometry. Apoptotic cells were stained with annexin‐V/fluorescein isothiocyanate (FITC) and propidium iodide (PI). Data are presented as the mean ± SD of three independent experiments. * p < 0.05 compared with DMSO.

    Journal: Thoracic Cancer

    Article Title: Effects of Autophagy Inhibition by SAR405 , a Selective VPS34 Inhibitor, on Pleural Mesothelioma Cells

    doi: 10.1111/1759-7714.70255

    Figure Lengend Snippet: Effects of SAR405 on apoptosis of H2452 pleural mesothelioma cells. (A) Mesothelioma H2452 cells were treated with 10.0 μM SAR405 for 48 h, stained with Hoechst 33342 to assess nuclear morphology, and visualized under a fluorescence microscope. Arrows indicate apoptotic cells. Scale bars = 200 μm. (B) Mesothelioma cells were treated with 2.5–10.0 μM SAR405 or dimethyl sulfoxide (DMSO) for 24 h and subjected to western blotting analysis to determine the expression of poly ADP‐ribose polymerase (PARP), cleaved (c‐) PARP, caspase‐3, c‐caspase‐3, and c‐caspase‐9. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase. (C) The induction of apoptosis by SAR405 was evaluated using flow cytometry. Apoptotic cells were stained with annexin‐V/fluorescein isothiocyanate (FITC) and propidium iodide (PI). Data are presented as the mean ± SD of three independent experiments. * p < 0.05 compared with DMSO.

    Article Snippet: The primary antibodies used in this study were as follows: caspase‐3 rabbit polyclonal antibody (pAb; 9662), cleaved caspase‐9 (Asp315) rabbit pAb (9505), poly (ADP‐ribose) polymerase (PARP) rabbit pAb (9542), glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH; D16H11) rabbit mAb (5174), and LC3B (D11) rabbit mAb (3868; all from Cell Signaling, Beverly, MA, USA); and p62 (sequestosome 1) rabbit pAb (PM045; MBL, Chiba, Japan).

    Techniques: Staining, Fluorescence, Microscopy, Western Blot, Expressing, Flow Cytometry